› 2fnj

X-ray diffraction
1.8Å resolution

Crystal structure of a B30.2/SPRY domain-containing protein GUSTAVUS in complex with Elongin B and Elongin C

Released:
DOI: 10.2210/pdb2fnj/pdb
PDB entry 2fnj coloured by chain, front view.
PDB entry 2fnj coloured by chain, side view.
PDB entry 2fnj coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional insights into the B30.2/SPRY domain.
Woo JS, Imm JH, Min CK, Kim KJ, Cha SS, Oh BH
EMBO J 25 1353-63 (2006)
PMID: 16498413

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-106772 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein gustavus Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.7 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: A1Z6E0 (Residues: 27-251; Coverage: 81%)
Gene names: CG2944, gus
Sequence domains:
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.19 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62869 (Residues: 1-118; Coverage: 100%)
Gene names: Elob, Tceb2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P83940 (Residues: 17-112; Coverage: 86%)
Gene names: Eloc, Tceb1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 50.308Å b: 82.489Å c: 118.966Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.351 0.218 0.247
Expression system: Escherichia coli

Quick links

Citations

26 review citations

The control of viral infection by tripartite motif proteins and cyclophilin A.
Towers GJ. (2007)
25 more

11 mentions without citation

Conserved structural and functional aspects of the tripartite motif gene family point towards therapeutic applications in multiple diseases.
Gushchina et al. (2018)
10 more