› 2fmt

X-ray diffraction
2.8Å resolution

METHIONYL-TRNAFMET FORMYLTRANSFERASE COMPLEXED WITH FORMYL-METHIONYL-TRNAFMET

Released:
DOI: 10.2210/pdb2fmt/pdb
PDB entry 2fmt coloured by chain, front view.
PDB entry 2fmt coloured by chain, side view.
PDB entry 2fmt coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of methionyl-tRNAfMet transformylase complexed with the initiator formyl-methionyl-tRNAfMet.
Schmitt E, Panvert M, Blanquet S, Mechulam Y
EMBO J 17 6819-26 (1998)
PMID: 9843487

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150094 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Methionyl-tRNA formyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 34.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P23882 (Residues: 2-315; Coverage: 100%)
Gene names: JW3249, b3288, fmt, yhdD
Sequence domains:
Structure domains:
FORMYL-METHIONYL-TRNAFMET2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 77 nucleotides
Theoretical weight: 24.83 KDa
Source organism: synthetic construct
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand FME 2 x FME
5 modified residues:
Ligand 4SU 2 x 4SU
Ligand H2U 2 x H2U
Ligand OMC 2 x OMC
Ligand 5MU 2 x 5MU
Ligand PSU 2 x PSU

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P21212
Unit cell:
a: 201.71Å b: 68.06Å c: 86.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.292
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Initiation of translation in prokaryotes and eukaryotes.
Kozak M. (1999)
9 more

20 mentions without citation

Initiation of protein synthesis in bacteria.
Laursen et al. (2005)
19 more