› 2fll

X-ray diffraction
2.6Å resolution

Ternary complex of human DNA polymerase iota with DNA and dTTP

Released:
DOI: 10.2210/pdb2fll/pdb
PDB entry 2fll coloured by chain, front view.
PDB entry 2fll coloured by chain, side view.
PDB entry 2fll coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An incoming nucleotide imposes an anti to syn conformational change on the templating purine in the human DNA polymerase-iota active site.
Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK
Structure 14 749-55 (2006)
PMID: 16615915

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115267 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase iota Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 47.03 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9UNA4 (Residues: 26-445; Coverage: 57%)
Gene names: POLI, RAD30B
Sequence domains:
Structure domains:
DNA primer strand Chain: P
Molecule details ›
Chain: P
Length: 7 nucleotides
Theoretical weight: 2.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA template strand Chain: T
Molecule details ›
Chain: T
Length: 11 nucleotides
Theoretical weight: 3.34 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand TTP 1 x TTP
1 modified residue:
Ligand DOC 1 x DOC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P6522
Unit cell:
a: 98.329Å b: 98.329Å c: 202.186Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.223 0.277
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided

Quick links

Citations

15 review citations

Translesion DNA polymerases.
Goodman et al. (2013)
14 more

6 mentions without citation

What a difference a decade makes: insights into translesion DNA synthesis.
Yang et al. (2007)
5 more