› 2f2s

X-ray diffraction
2Å resolution

Human mitochondrial acetoacetyl-CoA thiolase

Released:
DOI: 10.2210/pdb2f2s/pdb
PDB entry 2f2s coloured by chain, front view.
PDB entry 2f2s coloured by chain, side view.
PDB entry 2f2s coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Min JR, Dombrovski L, Antoshenko T, Wu H, Loppnau P, Weigelt J, Sundstrom M, Arrowsmith CH, Edwards AM, Bochkarev A, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 406 amino acids
Theoretical weight: 42.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P24752 (Residues: 41-427; Coverage: 91%)
Gene names: ACAT, ACAT1, MAT
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COA 4 x COA
1 bound ligand:
Ligand CL 3 x CL
1 modified residue:
Ligand SCY 4 x SCY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 56.989Å b: 126.64Å c: 111.858Å
α: 90° β: 98.64° γ: 90°
R-values:
R R work R free
0.202 0.199 0.257
Expression system: Escherichia coli

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