› 2f00

X-ray diffraction
2.5Å resolution

Escherichia coli MurC

Released:
DOI: 10.2210/pdb2f00/pdb
PDB entry 2f00 coloured by chain, front view.
PDB entry 2f00 coloured by chain, side view.
PDB entry 2f00 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of Escherichia coli UDP-N-acetylmuramoyl:L-alanine ligase (MurC).
Deva T, Baker EN, Squire CJ, Smith CA
Acta Crystallogr D Biol Crystallogr 62 1466-74 (2006)
PMID: 17139082

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramate--L-alanine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 491 amino acids
Theoretical weight: 54.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P17952 (Residues: 1-491; Coverage: 100%)
Gene names: JW0089, b0091, murC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 73.926Å b: 93.134Å c: 176.792Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.26
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
4 more

9 mentions without citation

Structure and function of the first full-length murein peptide ligase (Mpl) cell wall recycling protein.
Das et al. (2011)
8 more