› 2ext

X-ray diffraction
1.8Å resolution

TRAP4 (engineered TRAP)

Released:
DOI: 10.2210/pdb2ext/pdb
PDB entry 2ext coloured by chain, front view.
PDB entry 2ext coloured by chain, side view.
PDB entry 2ext coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Rounding up: Engineering 12-membered rings from the cyclic 11-mer TRAP.
Heddle JG, Yokoyama T, Yamashita I, Park SY, Tame JR
Structure 14 925-33 (2006)
PMID: 16698553

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription attenuation protein MtrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 77 amino acids
Theoretical weight: 8.47 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9X6J6 (Residues: 2-74; Coverage: 99%)
Gene name: mtrB
Sequence domains: Tryptophan RNA-binding attenuator protein
Structure domains: TRAP-like

Ligands and Environments

1 bound ligand:
Ligand TRP 3 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P4212
Unit cell:
a: 109.789Å b: 109.789Å c: 36.871Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.218
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Protein cages, rings and tubes: useful components of future nanodevices?
Heddle JG. (2008)
2 more

2 mentions without citation

Expression patterns of SP1 and SP3 during mouse spermatogenesis: SP1 down-regulation correlates with two successive promoter changes and translationally compromised transcripts.
Ma et al. (2008)
1 more