› 2ewy

X-ray diffraction
3.1Å resolution

Crystal structure of human BACE2 in complex with a hydroxyethylenamine transition-state inhibitor

Released:
DOI: 10.2210/pdb2ewy/pdb
PDB entry 2ewy coloured by chain, front view.
PDB entry 2ewy coloured by chain, side view.
PDB entry 2ewy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human BACE2 in complex with a hydroxyethylamine transition-state inhibitor.
Ostermann N, Eder J, Eidhoff U, Zink F, Hassiepen U, Worpenberg S, Maibaum J, Simic O, Hommel U, Gerhartz B
J Mol Biol 355 249-61 (2006)
PMID: 16305800

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195305 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-secretase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 383 amino acids
Theoretical weight: 41.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5Z0 (Residues: 78-460; Coverage: 77%)
Gene names: AEPLC, ALP56, ASP21, BACE2, CDA13, UNQ418/PRO852
Sequence domains: Eukaryotic aspartyl protease
Structure domains: Acid Proteases

Ligands and Environments

1 bound ligand:
Ligand DBO 4 x DBO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R3
Unit cell:
a: 228.407Å b: 228.407Å c: 108.964Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.227 0.247
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Proteases and proteolysis in Alzheimer disease: a multifactorial view on the disease process.
De Strooper B. (2010)
3 more

10 mentions without citation

BACE1 (β-secretase) inhibitors for the treatment of Alzheimer's disease.
Ghosh et al. (2014)
9 more