2evg

X-ray diffraction
1.55Å resolution

Structure of a Ndt80-DNA complex (MSE mutant mA7T)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-115220 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Meiosis-specific transcription factor NDT80 Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 39.59 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P38830 (Residues: 1-340; Coverage: 54%)
Gene names: DAS1, NDT80, YHR124W
Sequence domains: NDT80 / PhoG like DNA-binding family
Structure domains: NDT80 DNA-binding domain
5'-D(*TP*GP*CP*GP*AP*CP*AP*CP*AP*TP*AP*AP*AP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(*AP*GP*TP*TP*TP*AP*TP*GP*TP*GP*TP*CP*GP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.3 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 70.432Å b: 78.781Å c: 162.671Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.184
Expression systems:
  • Escherichia coli BL21
  • Not provided