› 2et7

X-ray diffraction
1.7Å resolution

Structural and spectroscopic insights into the mechanism of oxalate oxidase

Released:
DOI: 10.2210/pdb2et7/pdb
PDB entry 2et7 coloured by chain, front view.
PDB entry 2et7 coloured by chain, side view.
PDB entry 2et7 coloured by chain, top view.
Source organism: Hordeum vulgare
Primary publication:
Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase.
Opaleye O, Rose RS, Whittaker MM, Woo EJ, Whittaker JW, Pickersgill RW
J Biol Chem 281 6428-33 (2006)
PMID: 16291738

Function and Biology Details

Reaction catalysed: 
Oxalate + O(2) + 2 H(+) = 2 CO(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155354 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxalate oxidase 1 Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 21.18 KDa
Source organism: Hordeum vulgare
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P45850 (Residues: 1-201; Coverage: 100%)
Sequence domains: Cupin
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: R32
Unit cell:
a: 94.823Å b: 94.823Å c: 106.238Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.163 0.211
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Oxygen activation by mononuclear Mn, Co, and Ni centers in biology and synthetic complexes.
Fiedler et al. (2017)
1 more

3 mentions without citation

Non-homologous isofunctional enzymes: a systematic analysis of alternative solutions in enzyme evolution.
Omelchenko et al. (2010)
2 more