› 2es2

X-ray diffraction
1.78Å resolution

Crystal Structure Analysis of the Bacillus Subtilis Cold Shock Protein Bs-CspB in Complex with Hexathymidine

Released:
DOI: 10.2210/pdb2es2/pdb
PDB entry 2es2 coloured by chain, front view.
PDB entry 2es2 coloured by chain, side view.
PDB entry 2es2 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
T-rich DNA single strands bind to a preformed site on the bacterial cold shock protein Bs-CspB.
Max KE, Zeeb M, Bienert R, Balbach J, Heinemann U
J Mol Biol 360 702-14 (2006)
PMID: 16780871

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115212 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cold shock protein CspB Chain: A
Molecule details ›
Chain: A
Length: 67 amino acids
Theoretical weight: 7.37 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32081 (Residues: 1-67; Coverage: 100%)
Gene names: BSU09100, cspA, cspB
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains: Nucleic acid-binding proteins
5'-D(*TP*TP*TP*TP*TP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 6 nucleotides
Theoretical weight: 1.78 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 49.03Å b: 53.17Å c: 76.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.223
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
5 more

16 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
15 more