2e69

X-ray diffraction
2.2Å resolution

Crystal structure of the stationary phase survival protein SurE from Thermus thermophilus HB8 in complex with sulfate

Released:
Source organism: Thermus thermophilus HB8

Function and Biology Details

Reaction catalysed:
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-176164 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-nucleotidase SurE Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 244 amino acids
Theoretical weight: 26.63 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53W92 (Residues: 1-244; Coverage: 100%)
Gene names: TTHB070, surE
Sequence domains: Survival protein SurE
Structure domains: Survival protein SurE-like phosphatase/nucleotidase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P3121
Unit cell:
a: 131.825Å b: 131.825Å c: 131.209Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.195 0.23
Expression system: Escherichia coli BL21(DE3)