› 2e5x

X-ray diffraction
2Å resolution

Structure of nucleotide triphosphate pyrophosphatase from pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2e5x/pdb
PDB entry 2e5x coloured by chain, front view.
PDB entry 2e5x coloured by chain, side view.
PDB entry 2e5x coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Mizutani H, Lokanath NK, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ITP + H(2)O = IMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dITP/XTP pyrophosphatase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.23 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59580 (Residues: 1-186; Coverage: 100%)
Gene name: PH1917
Sequence domains: Ham1 family
Structure domains: Maf protein

Ligands and Environments

3 bound ligands:
Ligand NA 3 x NA
Ligand ITT 1 x ITT
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P3221
Unit cell:
a: 75.08Å b: 75.08Å c: 84.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.196 0.222
Expression system: Escherichia coli

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