› 2dr2

X-ray diffraction
3Å resolution

Structure of human tryptophanyl-tRNA synthetase in complex with tRNA(Trp)

Released:
DOI: 10.2210/pdb2dr2/pdb
PDB entry 2dr2 coloured by chain, front view.
PDB entry 2dr2 coloured by chain, side view.
PDB entry 2dr2 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of human tryptophanyl-tRNA synthetase in complex with tRNATrp reveals the molecular basis of tRNA recognition and specificity.
Shen N, Guo L, Yang B, Jin Y, Ding J
Nucleic Acids Res 34 3246-58 (2006)
PMID: 16798914

Function and Biology Details

Reaction catalysed: 
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149947 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
T2-TrpRS Chain: A
Molecule details ›
Chain: A
Length: 384 amino acids
Theoretical weight: 44.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23381 (Residues: 94-471; Coverage: 80%)
Gene names: IFI53, WARS, WARS1, WRS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:
transfer RNA-Trp Chain: B
Molecule details ›
Chain: B
Length: 75 nucleotides
Theoretical weight: 24.18 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
Sequence domains: tRNA

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand TRP 1 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P4322
Unit cell:
a: 132.3Å b: 132.3Å c: 137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.285
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The nonribosomal synthesis of diketopiperazines in tRNA-dependent cyclodipeptide synthase pathways.
Belin et al. (2012)
8 more

16 mentions without citation

Neurodegenerative Charcot-Marie-Tooth disease as a case study to decipher novel functions of aminoacyl-tRNA synthetases.
Wei et al. (2019)
15 more