› 2d48

X-ray diffraction
1.65Å resolution

Crystal structure of the Interleukin-4 variant T13D

Released:
DOI: 10.2210/pdb2d48/pdb
PDB entry 2d48 coloured by chain, front view.
PDB entry 2d48 coloured by chain, side view.
PDB entry 2d48 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A modular interface of IL-4 allows for scalable affinity without affecting specificity for the IL-4 receptor.
Kraich M, Klein M, Patiño E, Harrer H, Nickel J, Sebald W, Mueller TD
BMC Biol 4 13 (2006)
PMID: 16640778

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Interleukin-4 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05112 (Residues: 25-153; Coverage: 100%)
Gene name: IL4
Sequence domains: Interleukin 4
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 90.528Å b: 90.528Å c: 45.907Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.247
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Fundamental aspects of protein-protein association kinetics.
Schreiber et al. (2009)
16 more

3 mentions without citation

A modular interface of IL-4 allows for scalable affinity without affecting specificity for the IL-4 receptor.
Kraich et al. (2006)
2 more