› 2d3c

X-ray diffraction
3.81Å resolution

Crystal Structure of the Maize Glutamine Synthetase complexed with ADP and Phosphinothricin Phosphate

Released:
DOI: 10.2210/pdb2d3c/pdb
PDB entry 2d3c coloured by chain, front view.
PDB entry 2d3c coloured by chain, side view.
PDB entry 2d3c coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Atomic structure of plant glutamine synthetase: a key enzyme for plant productivity.
Unno H, Uchida T, Sugawara H, Kurisu G, Sugiyama T, Yamaya T, Sakakibara H, Hase T, Kusunoki M
J Biol Chem 281 29287-96 (2006)
PMID: 16829528

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153679 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine synthetase root isozyme 3 Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 356 amino acids
Theoretical weight: 39.26 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: P38561 (Residues: 1-356; Coverage: 100%)
Gene names: GLN4, GS1-3
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 30 x MN
Ligand P3P 10 x P3P
Ligand ADP 10 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P21
Unit cell:
a: 95.958Å b: 191.012Å c: 118.124Å
α: 90° β: 101.23° γ: 90°
R-values:
R R work R free
0.187 0.185 0.229
Expression system: Escherichia coli

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Citations

8 review citations

Nitrogen uptake, assimilation and remobilization in plants: challenges for sustainable and productive agriculture.
Masclaux-Daubresse et al. (2010)
7 more

2 mentions without citation

Assisted assignment of ligands corresponding to unknown electron density.
Binkowski et al. (2010)
1 more