› 2d31

X-ray diffraction
3.2Å resolution

Crystal structure of disulfide-linked HLA-G dimer

Released:
DOI: 10.2210/pdb2d31/pdb
PDB entry 2d31 coloured by chain, front view.
PDB entry 2d31 coloured by chain, side view.
PDB entry 2d31 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer.
Shiroishi M, Kuroki K, Ose T, Rasubala L, Shiratori I, Arase H, Tsumoto K, Kumagai I, Kohda D, Maenaka K
J Biol Chem 281 10439-47 (2006)
PMID: 16455647

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-147612 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, alpha chain G Chains: A, D
Molecule details ›
Chains: A, D
Length: 276 amino acids
Theoretical weight: 31.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17693 (Residues: 25-300; Coverage: 88%)
Gene names: HLA-6.0, HLA-G, HLAG
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Histone H2AX Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P16104 (Residues: 78-86; Coverage: 6%)
Gene names: H2AFX, H2AX

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21212
Unit cell:
a: 94.641Å b: 127.807Å c: 72.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.235 0.298
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

54 review citations

Immunology of placentation in eutherian mammals.
Moffett et al. (2006)
53 more

7 mentions without citation

Dimerization of Transmembrane Proteins in Cancer Immunotherapy.
Li et al. (2023)
6 more