› 2czu

X-ray diffraction
2.1Å resolution

lipocalin-type prostaglandin D synthase

Released:
DOI: 10.2210/pdb2czu/pdb
PDB entry 2czu coloured by chain, front view.
PDB entry 2czu coloured by chain, side view.
PDB entry 2czu coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of the catalytic mechanism operating in open-closed conformers of lipocalin type prostaglandin D synthase.
Kumasaka T, Aritake K, Ago H, Irikura D, Tsurumura T, Yamamoto M, Miyano M, Urade Y, Hayaishi O
J Biol Chem 284 22344-22352 (2009)
PMID: 19546224

Function and Biology Details

Reaction catalysed: 
(5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prostaglandin-H2 D-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 18.62 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O09114 (Residues: 25-189; Coverage: 100%)
Gene name: Ptgds
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P212121
Unit cell:
a: 46.2Å b: 66.8Å c: 105.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.279
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Prostaglandin synthases: Molecular characterization and involvement in prostaglandin biosynthesis.
Seo et al. (2017)
2 more

3 mentions without citation

Enzymes of the cyclooxygenase pathways of prostanoid biosynthesis.
Smith et al. (2011)
2 more