› 2cuu

X-ray diffraction
1.75Å resolution

Crystal structure of spin labeled T4 Lysozyme (V131R1)

Released:
DOI: 10.2210/pdb2cuu/pdb
PDB entry 2cuu coloured by chain, front view.
PDB entry 2cuu coloured by chain, side view.
PDB entry 2cuu coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Structural origin of weakly ordered nitroxide motion in spin-labeled proteins.
Fleissner MR, Cascio D, Hubbell WL
Protein Sci 18 893-908 (2009)
PMID: 19384990

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.63 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00720 (Residues: 1-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

4 bound ligands:
Ligand MTN 1 x MTN
Ligand AZI 1 x AZI
Ligand CL 3 x CL
Ligand HED 1 x HED
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: P3221
Unit cell:
a: 60.405Å b: 60.405Å c: 96.055Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.191 0.224
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Technological advances in site-directed spin labeling of proteins.
Hubbell et al. (2013)
6 more

8 mentions without citation

MtsslWizard: In Silico Spin-Labeling and Generation of Distance Distributions in PyMOL.
Hagelueken et al. (2012)
7 more