› 2csd

X-ray diffraction
2.9Å resolution

Crystal structure of Topoisomerase V (61 kDa fragment)

Released:
DOI: 10.2210/pdb2csd/pdb
PDB entry 2csd coloured by chain, front view.
PDB entry 2csd coloured by chain, side view.
PDB entry 2csd coloured by chain, top view.
Source organism: Methanopyrus kandleri
Primary publication:
Structure of the N-terminal fragment of topoisomerase V reveals a new family of topoisomerases.
Taneja B, Patel A, Slesarev A, Mondragón A
EMBO J 25 398-408 (2006)
PMID: 16395333

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Helix-hairpin-helix DNA-binding motif class 1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 60 KDa
Source organism: Methanopyrus kandleri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q977W1 (Residues: 1-519; Coverage: 53%)
Gene names: HA336_03250, top5
Sequence domains:
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P212121
Unit cell:
a: 75.5Å b: 86.4Å c: 175.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.236 0.308
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

All tangled up: how cells direct, manage and exploit topoisomerase function.
Vos et al. (2011)
12 more

2 mentions without citation

Phylogenomics of DNA topoisomerases: their origin and putative roles in the emergence of modern organisms.
Forterre et al. (2009)
1 more