› 2cht

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURES OF THE MONOFUNCTIONAL CHORISMATE MUTASE FROM BACILLUS SUBTILIS AND ITS COMPLEX WITH A TRANSITION STATE ANALOG

Released:
DOI: 10.2210/pdb2cht/pdb
PDB entry 2cht coloured by chain, front view.
PDB entry 2cht coloured by chain, side view.
PDB entry 2cht coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structures of the monofunctional chorismate mutase from Bacillus subtilis and its complex with a transition state analog.
Chook YM, Ke H, Lipscomb WN
Proc Natl Acad Sci U S A 90 8600-3 (1993)
PMID: 8378335

Function and Biology Details

Reaction catalysed: 
Chorismate = prephenate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chorismate mutase AroH Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 127 amino acids
Theoretical weight: 14.51 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
UniProt:
  • Canonical: P19080 (Residues: 1-127; Coverage: 100%)
Gene names: BSU22690, aroH
Sequence domains: Chorismate mutase type I
Structure domains: RutC-like

Ligands and Environments

1 bound ligand:
Ligand TSA 12 x TSA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 102.4Å b: 68.3Å c: 102.8Å
α: 90° β: 105.6° γ: 90°
R-values:
R R work R free
0.182 0.182 not available
Expression system: Not provided

Quick links

Citations

17 review citations

The shikimate pathway and aromatic amino Acid biosynthesis in plants.
Maeda et al. (2012)
16 more

15 mentions without citation

Absolute binding free energy calculations: on the accuracy of computational scoring of protein-ligand interactions.
Singh et al. (2010)
14 more