› 2cda

X-ray diffraction
2.28Å resolution

Sulfolobus solfataricus Glucose Dehydrogenase 1 in complex with NADP

Released:
DOI: 10.2210/pdb2cda/pdb
PDB entry 2cda coloured by chain, front view.
PDB entry 2cda coloured by chain, side view.
PDB entry 2cda coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
The structural basis of substrate promiscuity in glucose dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.
Milburn CC, Lamble HJ, Theodossis A, Bull SD, Hough DW, Danson MJ, Taylor GL
J Biol Chem 281 14796-804 (2006)
PMID: 16556607

Function and Biology Details

Reactions catalysed: 
D-galactose + NADP(+) = D-galactono-1,5-lactone + NADPH
An aldopyranose + NAD(P)(+) = an aldono-1,5-lactone + NAD(P)H 
D-glucose + NAD(P)(+) = D-glucono-1,5-lactone + NAD(P)H
D-galactose + NAD(+) = D-galactono-1,4-lactone + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose 1-dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 40.95 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: O93715 (Residues: 1-366; Coverage: 100%)
Gene name: gdh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 68.62Å b: 92.296Å c: 138.264Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.246
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
1 more

4 mentions without citation

KIT mutation analysis in mast cell neoplasms: recommendations of the European Competence Network on Mastocytosis.
Arock et al. (2015)
3 more