› 2c32

X-ray diffraction
7.01Å resolution

Co-axial association of recombinant eye lens aquaporin-0 observed in loosely packed 3D-crystals

Released:
DOI: 10.2210/pdb2c32/pdb
PDB entry 2c32 coloured by chain, front view.
PDB entry 2c32 coloured by chain, side view.
PDB entry 2c32 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Co-axial association of recombinant eye lens aquaporin-0 observed in loosely packed 3D crystals.
Palanivelu DV, Kozono DE, Engel A, Suda K, Lustig A, Agre P, Schirmer T
J Mol Biol 355 605-11 (2006)
PMID: 16309700

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lens fiber major intrinsic protein Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 28.24 KDa
Source organism: Bos taurus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P06624 (Residues: 1-263; Coverage: 100%)
Gene name: MIP
Sequence domains: Major intrinsic protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I432
Unit cell:
a: 188.271Å b: 188.271Å c: 188.271Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.39 0.39 0.387
Expression system: Saccharomyces cerevisiae

Quick links

Citations

5 review citations

Aquaporin gating.
Hedfalk et al. (2006)
4 more

5 mentions without citation

Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures.
Wlodawer et al. (2008)
4 more