› 2c24

X-ray diffraction
2.27Å resolution

FAMILY 30 CARBOHYDRATE-BINDING MODULE OF CELLULOSOMAL CELLULASE CEL9D- CEL44B OF CLOSTRIDIUM THERMOCELLUM

Released:
DOI: 10.2210/pdb2c24/pdb
PDB entry 2c24 coloured by chain, front view.
PDB entry 2c24 coloured by chain, side view.
PDB entry 2c24 coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Xyloglucan is recognized by carbohydrate-binding modules that interact with beta-glucan chains.
Najmudin S, Guerreiro CI, Carvalho AL, Prates JA, Correia MA, Alves VD, Ferreira LM, Romão MJ, Gilbert HJ, Bolam DN, Fontes CM
J Biol Chem 281 8815-28 (2006)
PMID: 16314409

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase J Chains: A, B
Molecule details ›
Chains: A, B
Length: 206 amino acids
Theoretical weight: 23.31 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli
UniProt:
  • Canonical: P71140 (Residues: 23-223; Coverage: 13%)
Gene name: celJ
Sequence domains: Carbohydrate binding domain 30
Structure domains: Galactose-binding lectin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 66.579Å b: 85.475Å c: 88.926Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.216 0.265
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.
Fontes et al. (2010)
7 more

2 mentions without citation

Emerging roles of circular RNAs in liver cancer.
Louis et al. (2022)
1 more