› 2byr

X-ray diffraction
2.45Å resolution

CRYSTAL STRUCTURE OF ACHBP FROM APLYSIA CALIFORNICA in complex with methyllycaconitine

Released:
DOI: 10.2210/pdb2byr/pdb
PDB entry 2byr coloured by chain, front view.
PDB entry 2byr coloured by chain, side view.
PDB entry 2byr coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations.
Hansen SB, Sulzenbacher G, Huxford T, Marchot P, Taylor P, Bourne Y
EMBO J 24 3635-46 (2005)
PMID: 16193063

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 227 amino acids
Theoretical weight: 25.85 KDa
Source organism: Aplysia californica
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8WSF8 (Residues: 18-236; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

1 bound ligand:
Ligand MLK 10 x MLK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 67.267Å b: 135.776Å c: 147.286Å
α: 90° β: 99.46° γ: 90°
R-values:
R R work R free
0.194 0.193 0.232
Expression system: Homo sapiens

Quick links

Citations

75 review citations

Mammalian nicotinic acetylcholine receptors: from structure to function.
Albuquerque et al. (2009)
74 more

27 mentions without citation

Structure-guided drug design: conferring selectivity among neuronal nicotinic receptor and acetylcholine-binding protein subtypes.
Taylor et al. (2007)
26 more