› 2boc

X-ray diffraction
3.01Å resolution

Potassium channel KcsA-Fab complex in thallium with tetraethylarsonium (TEAs)

Released:
DOI: 10.2210/pdb2boc/pdb
PDB entry 2boc coloured by chain, front view.
PDB entry 2boc coloured by chain, side view.
PDB entry 2boc coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of TEA blockade in a model potassium channel.
Lenaeus MJ, Vamvouka M, Focia PJ, Gross A
Nat Struct Mol Biol 12 454-9 (2005)
PMID: 15852022

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209450 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTIBODY FAB FRAGMENT HEAVY CHAIN Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
ANTIBODY FAB FRAGMENT LIGHT CHAIN Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.21 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 1-124; Coverage: 78%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand TL 4 x TL
Ligand CO 1 x CO
Ligand T1A 1 x T1A
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: I4
Unit cell:
a: 154.68Å b: 154.68Å c: 76.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.25
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Optochemical genetics.
Fehrentz et al. (2011)
11 more

4 mentions without citation

A cation-pi interaction between extracellular TEA and an aromatic residue in potassium channels.
Ahern et al. (2006)
3 more