› 2bnp

X-ray diffraction
2.7Å resolution

Lipidic cubic phase grown reaction centre from Rhodobacter sphaeroides, ground state

Released:
DOI: 10.2210/pdb2bnp/pdb
PDB entry 2bnp coloured by chain, front view.
PDB entry 2bnp coloured by chain, side view.
PDB entry 2bnp coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Conformational regulation of charge recombination reactions in a photosynthetic bacterial reaction center.
Katona G, Snijder A, Gourdon P, Andréasson U, Hansson O, Andréasson LE, Neutze R
Nat Struct Mol Biol 12 630-1 (2005)
PMID: 15937492

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein L chain Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: B
Molecule details ›
Chain: B
Length: 307 amino acids
Theoretical weight: 34.4 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein H chain Chain: C
Molecule details ›
Chain: C
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand BCL 4 x BCL
Ligand BPH 2 x BPH
Ligand MST 1 x MST
Ligand FE2 1 x FE2
Ligand U10 1 x U10
Ligand CL 1 x CL
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P42212
Unit cell:
a: 100.142Å b: 100.142Å c: 238.002Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.249

Quick links

Citations

4 review citations

Advances in kinetic protein crystallography.
Bourgeois et al. (2005)
3 more

3 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
2 more