› 2bmb

X-ray diffraction
2.3Å resolution

X-ray structure of the bifunctional 6-hydroxymethyl-7,8- dihydroxypterin pyrophosphokinase dihydropteroate synthase from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb2bmb/pdb
PDB entry 2bmb coloured by chain, front view.
PDB entry 2bmb coloured by chain, side view.
PDB entry 2bmb coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The three-dimensional structure of the bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase of Saccharomyces cerevisiae.
Lawrence MC, Iliades P, Fernley RT, Berglez J, Pilling PA, Macreadie IG
J Mol Biol 348 655-70 (2005)
PMID: 15826662

Function and Biology Details

Reactions catalysed: 
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate
7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Folic acid synthesis protein FOL1 Chain: A
Molecule details ›
Chain: A
Length: 545 amino acids
Theoretical weight: 61.7 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53848 (Residues: 293-824; Coverage: 65%)
Gene names: FOL1, N0848, YNL256W
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PMM 2 x PMM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P41212
Unit cell:
a: 92.566Å b: 92.566Å c: 192.401Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Targeting purine and pyrimidine metabolism in human apicomplexan parasites.
Hyde JE. (2007)
7 more

4 mentions without citation

BSSF: a fingerprint based ultrafast binding site similarity search and function analysis server.
Xiong et al. (2010)
3 more