› 2bem

X-ray diffraction
1.55Å resolution

Crystal structure of the Serratia marcescens chitin-binding protein CBP21

Released:
DOI: 10.2210/pdb2bem/pdb
PDB entry 2bem coloured by chain, front view.
PDB entry 2bem coloured by chain, side view.
PDB entry 2bem coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Crystal structure and binding properties of the Serratia marcescens chitin-binding protein CBP21.
Vaaje-Kolstad G, Houston DR, Riemen AH, Eijsink VG, van Aalten DM
J Biol Chem 280 11313-9 (2005)
PMID: 15590674

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitin-binding type-4 domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 170 amino acids
Theoretical weight: 18.81 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O83009 (Residues: 28-197; Coverage: 100%)
Gene names: AB868_00786, FHU12_0647, FOT62_12335, NCTC10211_02621, gbpA
Sequence domains: Lytic polysaccharide mono-oxygenase, cellulose-degrading
Structure domains: chitin-binding protein cbp21

Ligands and Environments

3 bound ligands:
Ligand EDO 15 x EDO
Ligand SO4 6 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 51.936Å b: 58.053Å c: 159.829Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.156 0.175
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

Bioethanol.
Gray et al. (2006)
25 more

32 mentions without citation

A structural overview of GH61 proteins - fungal cellulose degrading polysaccharide monooxygenases.
Lo Leggio et al. (2012)
31 more