› 2be9

X-ray diffraction
2.6Å resolution

Crystal structure of the CTP-liganded (T-State) aspartate transcarbamoylase from the extremely thermophilic archaeon Sulfolobus acidocaldarius

Released:
DOI: 10.2210/pdb2be9/pdb
PDB entry 2be9 coloured by chain, front view.
PDB entry 2be9 coloured by chain, side view.
PDB entry 2be9 coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius
Primary publication:
Crystal structure of Sulfolobus acidocaldarius aspartate carbamoyltransferase in complex with its allosteric activator CTP.
De Vos D, Xu Y, Aerts T, Van Petegem F, Van Beeumen JJ
Biochem Biophys Res Commun 372 40-4 (2008)
PMID: 18477471

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159949 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aspartate carbamoyltransferase catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 34.33 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55338 (Residues: 1-299; Coverage: 100%)
Gene names: Saci_1596, pyrB
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase
Aspartate carbamoyltransferase regulatory chain Chain: B
Molecule details ›
Chain: B
Length: 168 amino acids
Theoretical weight: 18.85 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli
UniProt:
  • Canonical: P74766 (Residues: 1-164; Coverage: 100%)
Gene names: Saci_1595, pyrI
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
Ligand CTP 1 x CTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P6322
Unit cell:
a: 131.932Å b: 131.932Å c: 139.132Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.223 0.271
Expression system: Escherichia coli

Quick links

Citations

1 review citation

From Genome to Structure and Back Again: A Family Portrait of the Transcarbamylases.
Shi et al. (2015)
1 other article

2 mentions without citation

Coupling dynamics and evolutionary information with structure to identify protein regulatory and functional binding sites.
Mishra et al. (2019)
1 more