› 2b7r

X-ray diffraction
1.7Å resolution

Structure of E378D mutant flavocytochrome c3

Released:
DOI: 10.2210/pdb2b7r/pdb
PDB entry 2b7r coloured by chain, front view.
PDB entry 2b7r coloured by chain, side view.
PDB entry 2b7r coloured by chain, top view.
Source organism: Shewanella frigidimarina
Primary publication:
A proton delivery pathway in the soluble fumarate reductase from Shewanella frigidimarina.
Pankhurst KL, Mowat CG, Rothery EL, Hudson JM, Jones AK, Miles CS, Walkinshaw MD, Armstrong FA, Reid GA, Chapman SK
J Biol Chem 281 20589-97 (2006)
PMID: 16699170

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate reductase (cytochrome) Chain: A
Molecule details ›
Chain: A
Length: 571 amino acids
Theoretical weight: 60.61 KDa
Source organism: Shewanella frigidimarina
Expression system: Shewanella frigidimarina
UniProt:
  • Canonical: P0C278 (Residues: 1-571; Coverage: 100%)
Gene names: fcc3, fccA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand HEM 4 x HEM
2 bound ligands:
Ligand NA 1 x NA
Ligand FUM 1 x FUM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 45.961Å b: 92.879Å c: 79.486Å
α: 90° β: 91.17° γ: 90°
R-values:
R R work R free
0.158 0.156 0.18
Expression system: Shewanella frigidimarina

Quick links

Citations

2 review citations

Catalytic mechanisms of complex II enzymes: a structural perspective.
Iverson TM. (2013)
1 more

2 mentions without citation

Crystallographic studies of the binding of ligands to the dicarboxylate site of Complex II, and the identity of the ligand in the "oxaloacetate-inhibited" state.
Huang et al. (2006)
1 more