› 2au3

X-ray diffraction
2Å resolution

Crystal Structure of the Aquifex aeolicus primase (Zinc Binding and RNA Polymerase Domains)

Released:
DOI: 10.2210/pdb2au3/pdb
PDB entry 2au3 coloured by chain, front view.
PDB entry 2au3 coloured by chain, side view.
PDB entry 2au3 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Crosstalk between primase subunits can act to regulate primer synthesis in trans.
Corn JE, Pease PJ, Hura GL, Berger JM
Mol Cell 20 391-401 (2005)
PMID: 16285921

Function and Biology Details

Reaction catalysed: 
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase Chain: A
Molecule details ›
Chain: A
Length: 407 amino acids
Theoretical weight: 46.69 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67465 (Residues: 1-405; Coverage: 81%)
Gene names: aq_1493, dnaG
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 43.222Å b: 66.403Å c: 138.157Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.238
Expression system: Escherichia coli

Quick links

Citations

12 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
11 more

16 mentions without citation

Mechanism and evolution of DNA primases.
Kuchta et al. (2010)
15 more