› 2apj

X-ray diffraction
1.6Å resolution

X-Ray Structure of Protein from Arabidopsis Thaliana AT4G34215 at 1.6 Angstrom Resolution

Released:
DOI: 10.2210/pdb2apj/pdb
PDB entry 2apj coloured by chain, front view.
PDB entry 2apj coloured by chain, side view.
PDB entry 2apj coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The structure at 1.6 Angstroms resolution of the protein product of the At4g34215 gene from Arabidopsis thaliana.
Bitto E, Bingman CA, McCoy JG, Allard ST, Wesenberg GE, Phillips GN
Acta Crystallogr D Biol Crystallogr 61 1655-61 (2005)
PMID: 16301800

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185269 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable carbohydrate esterase At4g34215 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 260 amino acids
Theoretical weight: 28.55 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8L9J9 (Residues: 1-260; Coverage: 100%)
Gene names: At4g34215, F10M10.12, F28A23_20
Sequence domains: Carbohydrate esterase, sialic acid-specific acetylesterase
Structure domains: SGNH hydrolase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEB 4 x SEB

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 40.688Å b: 71.918Å c: 93.213Å
α: 108.16° β: 93.25° γ: 90.4°
R-values:
R R work R free
0.147 0.146 0.183
Expression system: Escherichia coli

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Citations

2 review citations

Phylogeny, classification and metagenomic bioprospecting of microbial acetyl xylan esterases.
Adesioye et al. (2016)
1 more

8 mentions without citation

The SGNH hydrolase family: a template for carbohydrate diversity.
Anderson et al. (2022)
7 more