› 2anr

X-ray diffraction
1.94Å resolution

Crystal structure (II) of Nova-1 KH1/KH2 domain tandem with 25nt RNA hairpin

Released:
DOI: 10.2210/pdb2anr/pdb
PDB entry 2anr coloured by chain, front view.
PDB entry 2anr coloured by chain, side view.
PDB entry 2anr coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Protein-RNA and protein-protein recognition by dual KH1/2 domains of the neuronal splicing factor Nova-1.
Teplova M, Malinina L, Darnell JC, Song J, Lu M, Abagyan R, Musunuru K, Teplov A, Burley SK, Darnell RB, Patel DJ
Structure 19 930-44 (2011)
PMID: 21742260

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156347 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-binding protein Nova-1 Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 19.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P51513 (Residues: 49-246; Coverage: 34%)
Gene name: NOVA1
Sequence domains: KH domain
Structure domains: K Homology domain, type 1
5'-R(*CP*(5BU)P*CP*GP*CP*GP*GP*AP*UP*CP*AP*GP*UP*CP*AP*CP*CP*CP*AP*AP*GP*CP*GP*AP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 8.09 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand K 1 x K
Ligand MG 2 x MG
2 modified residues:
Ligand 5BU 1 x 5BU
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 159.453Å b: 37.885Å c: 38.523Å
α: 90° β: 96.64° γ: 90°
R-values:
R R work R free
0.23 0.227 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

RNA protein interaction in neurons.
Darnell RB. (2013)
7 more

12 mentions without citation

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
11 more