› 2an0

X-ray diffraction
2.6Å resolution

Crystal Structure of the P332G mutant of the Bacillus subtilis NOS

Released:
DOI: 10.2210/pdb2an0/pdb
PDB entry 2an0 coloured by chain, front view.
PDB entry 2an0 coloured by chain, side view.
PDB entry 2an0 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure of a loose dimer: an intermediate in nitric oxide synthase assembly.
Pant K, Crane BR
J Mol Biol 352 932-40 (2005)
PMID: 16126221

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 reduced flavodoxin + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 oxidized flavodoxin + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase oxygenase Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.48 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34453 (Residues: 6-363; Coverage: 98%)
Gene names: BSU07630, nos, yflM
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P21212
Unit cell:
a: 80.971Å b: 92.479Å c: 62.839Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.253
Expression system: Escherichia coli BL21(DE3)

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Citations

10 review citations

Bacterial nitric oxide synthases.
Crane et al. (2010)
9 more