› 2al1

X-ray diffraction
1.5Å resolution

Crystal Structure Analysis of Enolase Mg Subunit Complex at pH 8.0

Released:
DOI: 10.2210/pdb2al1/pdb
PDB entry 2al1 coloured by chain, front view.
PDB entry 2al1 coloured by chain, side view.
PDB entry 2al1 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and catalytic properties of an engineered heterodimer of enolase composed of one active and one inactive subunit.
Sims PA, Menefee AL, Larsen TM, Mansoorabadi SO, Reed GH
J Mol Biol 355 422-31 (2006)
PMID: 16309698

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 46.73 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00924 (Residues: 2-437; Coverage: 100%)
Gene names: ENO1, ENOA, G9160, HSP48, YGR254W
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 4 x MG
Ligand PEP 2 x PEP
Ligand 2PG 1 x 2PG
Ligand CL 1 x CL
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P21
Unit cell:
a: 72Å b: 65Å c: 85.9Å
α: 90° β: 99.5° γ: 90°
R-values:
R R work R free
0.153 0.153 0.217

Quick links

Citations

11 citation in other articles

Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases.
Szczepek et al. (2007)
10 more

8 mentions without citation

Redox-based reagents for chemoselective methionine bioconjugation.
Lin et al. (2017)
7 more