› 2ai9

X-ray diffraction
2.5Å resolution

S.aureus Polypeptide Deformylase

Released:
DOI: 10.2210/pdb2ai9/pdb
PDB entry 2ai9 coloured by chain, front view.
PDB entry 2ai9 coloured by chain, side view.
PDB entry 2ai9 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural variation and inhibitor binding in polypeptide deformylase from four different bacterial species.
Smith KJ, Petit CM, Aubart K, Smyth M, McManus E, Jones J, Fosberry A, Lewis C, Lonetto M, Christensen SB
Protein Sci 12 349-60 (2003)
PMID: 12538898

Function and Biology Details

Reaction catalysed: 
Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide deformylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.59 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68826 (Residues: 1-183; Coverage: 100%)
Gene names: def, def1, pdf1
Sequence domains: Polypeptide deformylase
Structure domains: Peptide deformylase

Ligands and Environments

2 bound ligands:
Ligand NI 2 x NI
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I4
Unit cell:
a: 167.3Å b: 167.3Å c: 42.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.272
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Impact of the N-terminal amino acid on targeted protein degradation.
Meinnel et al. (2006)
2 more