› 2a89

X-ray diffraction
1.85Å resolution

Monomeric Sarcosine Oxidase: Structure of a covalently flavinylated amine oxidizing enzyme

Released:
DOI: 10.2210/pdb2a89/pdb
PDB entry 2a89 coloured by chain, front view.
PDB entry 2a89 coloured by chain, side view.
PDB entry 2a89 coloured by chain, top view.
Source organism: Bacillus sp. B-0618
Primary publication:
Structure of the sodium borohydride-reduced N-(cyclopropyl)glycine adduct of the flavoenzyme monomeric sarcosine oxidase.
Chen ZW, Zhao G, Martinovic S, Jorns MS, Mathews FS
Biochemistry 44 15444-50 (2005)
PMID: 16300392

Function and Biology Details

Reaction catalysed: 
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric sarcosine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.1 KDa
Source organism: Bacillus sp. B-0618
Expression system: Escherichia coli
UniProt:
  • Canonical: P40859 (Residues: 2-390; Coverage: 100%)
Gene names: sox, soxA
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FCG 2 x FCG
2 bound ligands:
Ligand CL 3 x CL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 72.279Å b: 69.059Å c: 74.127Å
α: 90° β: 94.13° γ: 90°
R-values:
R R work R free
0.178 0.178 0.221
Expression system: Escherichia coli

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Citations

3 review citations

Inhibitors of Protein Methyltransferases and Demethylases.
Kaniskan et al. (2018)
2 more

1 mention without citation

Recreating the natural evolutionary trend in key microdomains provides an effective strategy for engineering of a thermomicrobial N-demethylase.
Xin et al. (2022)