› 264d

X-ray diffraction
2.44Å resolution

THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE A-TRACT DNA DODECAMER D(CGCAAATTTGCG) COMPLEXED WITH THE MINOR-GROOVE-BINDING DRUG HOECHST 33258

Released:
DOI: 10.2210/pdb264d/pdb
PDB entry 264d coloured by chain, front view.
PDB entry 264d coloured by chain, side view.
PDB entry 264d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Three-dimensional crystal structure of the A-tract DNA dodecamer d(CGCAAATTTGCG) complexed with the minor-groove-binding drug Hoechst 33258.
Vega MC, García Sáez I, Aymamí J, Eritja R, Van der Marel GA, Van Boom JH, Rich A, Coll M
Eur J Biochem 222 721-6 (1994)
PMID: 7517864

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115023 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*GP*CP*AP*AP*AP*TP*TP*TP*GP*CP*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand HT 1 x HT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-21
Spacegroup: P212121
Unit cell:
a: 25.5Å b: 41.4Å c: 65.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 not available
Expression system: Not provided

Quick links

Citations

4 review citations

Synthetic DNA minor groove-binding drugs.
Reddy et al. (1999)
3 more

9 mentions without citation

Water at DNA surfaces: ultrafast dynamics in minor groove recognition.
Pal et al. (2003)
8 more