1zwx

X-ray diffraction
1.9Å resolution

Crystal Structure of SmcL

Released:

Function and Biology Details

Reaction catalysed:
Sphingomyelin + H(2)O = N-acylsphingosine + phosphocholine
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-193199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sphingomyelinase C Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 34.5 KDa
Source organism: Listeria ivanovii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RLV9 (Residues: 35-335; Coverage: 98%)
Gene name: smcL
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P3121
Unit cell:
a: 66.86Å b: 66.86Å c: 180.848Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.233
Expression system: Escherichia coli BL21(DE3)