› 1zn2

X-ray diffraction
2.91Å resolution

Low Resolution Structure of Response Regulator StyR

Released:
DOI: 10.2210/pdb1zn2/pdb
PDB entry 1zn2 coloured by chain, front view.
PDB entry 1zn2 coloured by chain, side view.
PDB entry 1zn2 coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
An active-like structure in the unphosphorylated StyR response regulator suggests a phosphorylation- dependent allosteric activation mechanism.
Milani M, Leoni L, Rampioni G, Zennaro E, Ascenzi P, Bolognesi M
Structure 13 1289-97 (2005)
PMID: 16154086

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulatory protein Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.4 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: O30989 (Residues: 1-208; Coverage: 100%)
Gene name: styR
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 41.66Å b: 36.546Å c: 75.12Å
α: 90° β: 100.32° γ: 90°
R-values:
R R work R free
0.269 0.265 0.352
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Bacterial response regulators: versatile regulatory strategies from common domains.
Gao et al. (2007)
2 more

3 mentions without citation

Molecular strategies for phosphorylation-mediated regulation of response regulator activity.
Gao et al. (2010)
2 more