› 1zjb

X-ray diffraction
1.8Å resolution

Crystal structure of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45 (monoclinic form)

Released:
DOI: 10.2210/pdb1zjb/pdb
PDB entry 1zjb coloured by chain, front view.
PDB entry 1zjb coloured by chain, side view.
PDB entry 1zjb coloured by chain, top view.
Source organism: Burkholderia ubonensis subsp. mesacidophila
Primary publication:
Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45.
Ravaud S, Watzlawick H, Haser R, Mattes R, Aghajari N
Acta Crystallogr Sect F Struct Biol Cryst Commun 61 100-3 (2005)
PMID: 16508103

Function and Biology Details

Reaction catalysed: 
Sucrose = 6-O-alpha-D-glucopyranosyl-D-fructofuranose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 557 amino acids
Theoretical weight: 63.94 KDa
Source organism: Burkholderia ubonensis subsp. mesacidophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2PS28 (Residues: 28-584; Coverage: 99%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 63.71Å b: 85.95Å c: 119.69Å
α: 90° β: 97.71° γ: 90°
R-values:
R R work R free
0.178 0.178 0.205
Expression system: Escherichia coli

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Citations

1 review citation

Analyzing Current Trends and Possible Strategies to Improve Sucrose Isomerases' Thermostability.
Sardiña-Peña et al. (2023)
1 other article