› 1zhy

X-ray diffraction
1.6Å resolution

Structure of yeast oxysterol binding protein Osh4 in complex with cholesterol

Released:
DOI: 10.2210/pdb1zhy/pdb
PDB entry 1zhy coloured by chain, front view.
PDB entry 1zhy coloured by chain, side view.
PDB entry 1zhy coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural mechanism for sterol sensing and transport by OSBP-related proteins.
Im YJ, Raychaudhuri S, Prinz WA, Hurley JH
Nature 437 154-8 (2005)
PMID: 16136145

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxysterol-binding protein homolog 4 Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 49.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35844 (Residues: 2-434; Coverage: 100%)
Gene names: BSR3, KES1, LPI3C, OSH4, P2614, YPL145C
Sequence domains: Oxysterol-binding protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PB 2 x PB
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 82.76Å b: 94.21Å c: 65.412Å
α: 90° β: 96.27° γ: 90°
R-values:
R R work R free
0.223 0.216 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

85 review citations

Structure and function of ER membrane contact sites with other organelles.
Phillips et al. (2016)
84 more

13 mentions without citation

Advances on the Transfer of Lipids by Lipid Transfer Proteins.
Wong et al. (2017)
12 more