› 1zhg

X-ray diffraction
2.4Å resolution

Crystal structure of Beta-Hydroxyacyl-Acyl Carrier Protein Dehydratase (FabZ) from Plasmodium falciparum

Released:
DOI: 10.2210/pdb1zhg/pdb
PDB entry 1zhg coloured by chain, front view.
PDB entry 1zhg coloured by chain, side view.
PDB entry 1zhg coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Crystal structure of dimeric FabZ of Plasmodium falciparum reveals conformational switching to active hexamers by peptide flips.
Swarnamukhi PL, Sharma SK, Bajaj P, Surolia N, Surolia A, Suguna K
FEBS Lett 580 2653-60 (2006)
PMID: 16643907

Function and Biology Details

Reaction catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyacyl-[acyl-carrier-protein] dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 15.35 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q965D7 (Residues: 91-229; Coverage: 66%)
Gene name: fabZ
Sequence domains: FabA-like domain
Structure domains: Hotdog Thioesterase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I222
Unit cell:
a: 71.544Å b: 82.576Å c: 96.469Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Gates of enzymes.
Gora et al. (2013)
7 more

3 mentions without citation

Active site comparisons and catalytic mechanisms of the hot dog superfamily.
Labonte et al. (2013)
2 more