› 1zfp

X-ray diffraction
1.8Å resolution

GROWTH FACTOR RECEPTOR BINDING PROTEIN SH2 DOMAIN COMPLEXED WITH A PHOSPHOTYROSYL PENTAPEPTIDE

Released:
DOI: 10.2210/pdb1zfp/pdb
PDB entry 1zfp coloured by chain, front view.
PDB entry 1zfp coloured by chain, side view.
PDB entry 1zfp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the high affinity of amino-aromatic SH2 phosphopeptide ligands.
Rahuel J, García-Echeverría C, Furet P, Strauss A, Caravatti G, Fretz H, Schoepfer J, Gay B
J Mol Biol 279 1013-22 (1998)
PMID: 9642078

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-158858 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Growth factor receptor-bound protein 2 Chain: E
Molecule details ›
Chain: E
Length: 98 amino acids
Theoretical weight: 11.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62993 (Residues: 56-153; Coverage: 45%)
Gene names: ASH, GRB2
Sequence domains: SH2 domain
Structure domains: SH2 domain
EPIDERMAL GROWTH FACTOR RECEPTOR-DERIVED PEPTIDE Chain: I
Molecule details ›
Chain: I
Length: 7 amino acids
Theoretical weight: 863 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
2 modified residues:
Ligand BE2 1 x BE2
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: C2221
Unit cell:
a: 49.9Å b: 113.7Å c: 46.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 not available not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Progress towards the development of SH2 domain inhibitors.
Kraskouskaya et al. (2013)
3 more

7 mentions without citation

Quantitative prediction of protein-protein binding affinity with a potential of mean force considering volume correction.
Su et al. (2009)
6 more