› 1zcl

X-ray diffraction
2.9Å resolution

prl-1 c104s mutant in complex with sulfate

Released:
DOI: 10.2210/pdb1zcl/pdb
PDB entry 1zcl coloured by chain, front view.
PDB entry 1zcl coloured by chain, side view.
PDB entry 1zcl coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure and biochemical properties of PRL-1, a phosphatase implicated in cell growth, differentiation, and tumor invasion.
Sun JP, Wang WQ, Yang H, Liu S, Liang F, Fedorov AA, Almo SC, Zhang ZY
Biochemistry 44 12009-21 (2005)
PMID: 16142898

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein tyrosine phosphatase type IVA 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.53 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q78EG7 (Residues: 1-160; Coverage: 93%)
Gene names: Prl1, Ptp4a1
Sequence domains: Dual specificity phosphatase, catalytic domain
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: I213
Unit cell:
a: 146.681Å b: 146.681Å c: 146.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.261 0.296
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

Protein tyrosine phosphatases as potential therapeutic targets.
He et al. (2014)
16 more

3 mentions without citation

Targeting phosphatases of regenerating liver (PRLs) in cancer.
Wei et al. (2018)
2 more