› 1zay

X-ray diffraction
2.7Å resolution

PURINE REPRESSOR-HYPOXANTHINE-MODIFIED-PURF-OPERATOR COMPLEX

Released:
DOI: 10.2210/pdb1zay/pdb
PDB entry 1zay coloured by chain, front view.
PDB entry 1zay coloured by chain, side view.
PDB entry 1zay coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Zheleznova EE, Brennan RG

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114887 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH-type transcriptional repressor PurR Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 38.09 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACP7 (Residues: 2-341; Coverage: 100%)
Gene names: JW1650, b1658, purR
Sequence domains:
Structure domains:
DNA (5'-D(*AP*AP*CP*GP*AP*AP*AP*AP*TP*(1AP)P*TP*TP*TP*TP*CP*GP*T)-3') Chain: M
Molecule details ›
Chain: M
Length: 17 nucleotides
Theoretical weight: 5.22 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand HPA 1 x HPA
1 modified residue:
Ligand 1AP 1 x 1AP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2221
Unit cell:
a: 176.449Å b: 95.187Å c: 81.777Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 not available
Expression systems:
  • Escherichia coli
  • Not provided

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