› 1zao

X-ray diffraction
1.84Å resolution

Crystal Structure of A.fulgidus Rio2 Kinase Complexed With ATP and Manganese Ions

Released:
DOI: 10.2210/pdb1zao/pdb
PDB entry 1zao coloured by chain, front view.
PDB entry 1zao coloured by chain, side view.
PDB entry 1zao coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Autophosphorylation of Archaeoglobus fulgidus Rio2 and crystal structures of its nucleotide-metal ion complexes.
LaRonde-LeBlanc N, Guszczynski T, Copeland T, Wlodawer A
FEBS J 272 2800-10 (2005)
PMID: 15943813

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RIO-type serine/threonine-protein kinase Rio2 Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 32.86 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30245 (Residues: 1-282; Coverage: 100%)
Gene names: AF_2426, rio2
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand PO4 1 x PO4
Ligand ATP 1 x ATP
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 116.862Å b: 44.365Å c: 62.793Å
α: 90° β: 94.01° γ: 90°
R-values:
R R work R free
0.184 0.184 0.212
Expression system: Escherichia coli

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Citations

4 review citations

Powering through ribosome assembly.
Strunk et al. (2009)
3 more

8 mentions without citation

The ancient microbial RIO kinases.
LaRonde NA. (2014)
7 more