› 1z9k

X-ray diffraction
4.6Å resolution

Photosynthetic Reaction Center from Rhodobacter sphaeroides

Released:
DOI: 10.2210/pdb1z9k/pdb
PDB entry 1z9k coloured by chain, front view.
PDB entry 1z9k coloured by chain, side view.
PDB entry 1z9k coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Design of a redox-linked active metal site: manganese bound to bacterial reaction centers at a site resembling that of photosystem II.
Thielges M, Uyeda G, Cámara-Artigas A, Kálmán L, Williams JC, Allen JP
Biochemistry 44 7389-94 (2005)
PMID: 15895982

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein L chain Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Reaction center protein M chain Chain: B
Molecule details ›
Chain: B
Length: 307 amino acids
Theoretical weight: 34.4 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Reaction center protein H chain Chain: C
Molecule details ›
Chain: C
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand BCL 4 x BCL
Ligand BPH 2 x BPH
Ligand U10 2 x U10
Ligand FE 1 x FE
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P4222
Unit cell:
a: 207.8Å b: 207.8Å c: 107.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.34 0.33 0.33
Expression system: Cereibacter sphaeroides

Quick links

Citations

8 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
7 more

1 mention without citation

Structure of the dimeric RC-LH1-PufX complex from Rhodobaca bogoriensis investigated by electron microscopy.
Semchonok et al. (2012)