1yyo Summary

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Crystal structure of RNase III mutant E110K from Aquifex aeolicus complexed with double-stranded RNA at 2.9-Angstrom Resolution

The structure was published by Gan, J., Tropea, J.E., Austin, B.P., Court, D.L., Waugh, D.S., and Ji, X., in 2005 in a paper entitled "Intermediate states of ribonuclease III in complex with double-stranded RNA" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.9 Å and deposited in 2005.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely 5'-R(*CP*GP*CP*GP*AP*AP*UP*UP*CP*GP*CP*G)-3' and Ribonuclease III.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterohexamers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Ribonuclease III O67082 (1-221) (RNC_AQUAE)search Aquifex aeolicus VF5search 100% 221 99%
B Ribonuclease III O67082 (1-221) (RNC_AQUAE)search Aquifex aeolicus VF5search 100% 221 99%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
O67082 (1 - 221) Ribonuclease III Aquifex aeolicus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B (O67082) RNase III catalytic domain-likesearch, Double-stranded RNA-binding domain (dsRBD)search Ribonuclease iii, N-terminal Endonuclease Domain; Chain Asearch, Double Stranded RNA Binding Domainsearch PF00035: Double-stranded RNA binding motifsearch, PF00636: Ribonuclease III domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B (O67082) metal ion bindingsearch RNA bindingsearch ribonuclease III activitysearch hydrolase activitysearch nuclease activitysearch endonuclease activitysearch RNA processingsearch rRNA catabolic processsearch mRNA processingsearch rRNA processingsearch nucleic acid phosphodiester bond hydrolysissearch tRNA processingsearch cytoplasmsearch

Chain InterPro annotation
A, B Ribonuclease III domainsearch search Ribonuclease IIIsearch Double-stranded RNA-binding domainsearch