› 1yv0

X-ray diffraction
7Å resolution

Crystal structure of skeletal muscle troponin in the Ca2+-free state

Released:
DOI: 10.2210/pdb1yv0/pdb
PDB entry 1yv0 coloured by chain, front view.
PDB entry 1yv0 coloured by chain, side view.
PDB entry 1yv0 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Ca(2+)-regulated structural changes in troponin.
Vinogradova MV, Stone DB, Malanina GG, Karatzaferi C, Cooke R, Mendelson RA, Fletterick RJ
Proc Natl Acad Sci U S A 102 5038-43 (2005)
PMID: 15784741

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-135943 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Troponin T, fast skeletal muscle isoforms Chain: T
Molecule details ›
Chain: T
Length: 107 amino acids
Theoretical weight: 12.69 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12620 (Residues: 157-263; Coverage: 41%)
Gene name: TNNT3
Sequence domains: Troponin
Troponin I, fast skeletal muscle Chain: I
Molecule details ›
Chain: I
Length: 137 amino acids
Theoretical weight: 15.85 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68246 (Residues: 2-138; Coverage: 75%)
Gene name: TNNI2
Sequence domains: Troponin
Troponin C, skeletal muscle Chain: C
Molecule details ›
Chain: C
Length: 162 amino acids
Theoretical weight: 18.26 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02588 (Residues: 2-163; Coverage: 99%)
Gene name: TNNC2
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P43212
Unit cell:
a: 134.662Å b: 134.662Å c: 102.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.358 0.358 0.359
Expression system: Escherichia coli

Quick links

Citations

39 review citations

Structural basis for diversity of the EF-hand calcium-binding proteins.
Grabarek Z. (2006)
38 more

15 mentions without citation

Troponin structure and function: a view of recent progress.
Marston et al. (2020)
14 more